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ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. Duffy antigens are rendered inactive by enzyme treatments since the enzyme will remove the sialic acid from the RBC membrane. This process destroys: M, N, S, s, and Duffy antigens. The process enhances the reactions for Rh, Kidd, Lewis, I, and P antigens.
When performing an antibody panel, which one of the following antigens will be rendered inactive by enzyme treatment?

A) anti-Lua
B) anti-E
C) anti-Jka
D) anti-Fya

2. Which of the procedures listed below will increase the platelet concentration in the preparation of Platelets?

A) Centrifuge the blood at a high speed, remove the plasma and spin the plasma again at a high speed.
B) Centrifuge the blood at a low speed, remove the plasma and spin the plasma again at a low speed.
C) Centrifuge the bood at a low speed, remove the plasma and spin the plasma again at a high speed.
D) Increase the duration of rotation

3. Unexplained bleeding is associated with immediate hemolytic transfusion reactions, but is not usually associated with delayed hemolytic transfusion reactions. The bleeding results from disseminated intravascular coagulation (DIC) due to ABO antibodies causing intraventricular hemorrhage (IVH).
Which of the following signs and symptoms may be associated with immediate transfusion reaction, but is NOT usually associated with delayed hemolytic transfusion reaction?

A) Unexplained bleeding from surgical site
B) Transient jaundice
C) Fever and chills
D) Unexplained drop in hemoglobin

4. AHG must be added to the cells immediately following washing. Antibodies may elute from the cells if the cells are allowed to sit in saline without the addition of AHG.
Which one of the following may cause a FALSE-NEGATIVE result with antiglobulin techniques?

A) Red cell/AHG test sample is over-centrifuged
B) Saline used for washing the test sample has been stored in squirt bottles
C) Patient's red cells have an antibody coating them
D) Addition of AHG is delayed for 40 minutes or more after final saline wash
E) Patient's blood specimen was collected in silicone gel tubes

5. As maturation continues in the granulocytic series the nucleus of the metamyelocyte becomes kidney or bean shaped.
Identify the cell in this illustration indicated by the arrow: